1JXS

Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor

Summary for 1JXS

• Entry DOI: 10.2210/pdb1jxs/pdb
• NMR Information: BMRB: 4829
• Descriptor: interleukin enhancer binding factor (1 entity in total)
• Functional Keywords: dna-binding domain, winged helix, dna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus (Probable): Q01167
• Total number of polymer chains: 1
• Total formula weight: 11562.14

Authors

Chuang, W.J.,Liu, P.P.,Li, C.,Hsieh, Y.H.,Chen, S.W.,Chen, S.H.,Jeng, W.Y. (deposition date: 2001-09-08, release date: 2003-03-11, Last modification date: 2024-05-29)

Primary citation

Liu, P.P.,Chen, Y.C.,Li, C.,Hsieh, Y.H.,Chen, S.W.,Chen, S.H.,Jeng, W.Y.,Chuang, W.J.
Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)
PROTEINS: STRUCT.,FUNCT.,GENET., 49:543-553, 2002

PubMed Abstract: Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra alpha-helix. This is the first study to report the presence of a C-terminal alpha-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding.

PubMed: 12402362
DOI: 10.1002/prot.10227

Experimental method

SOLUTION NMR