1JXG

The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge

Summary for 1JXG

• Entry DOI: 10.2210/pdb1jxg/pdb
• Descriptor: PLASTOCYANIN A, COPPER (I) ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: beta barrel, photosynthesis
• Biological source: Populus nigra
• Cellular location: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P00299
• Total number of polymer chains: 2
• Total formula weight: 21400.79

Authors

Milani, M.,Andolfi, L.,Cannistraro, S.,Verbeet, M.P.,Bolognesi, M. (deposition date: 2001-09-07, release date: 2001-12-19, Last modification date: 2024-11-06)

Primary citation

Milani, M.,Andolfi, L.,Cannistraro, S.,Verbeet, M.P.,Bolognesi, M.
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.
Acta Crystallogr.,Sect.D, 57:1735-1738, 2001

PubMed Abstract: Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.

PubMed: 11679761
DOI: 10.1107/S0907444901013221

Experimental method

X-RAY DIFFRACTION (1.6 Å)