1JPM

L-Ala-D/L-Glu Epimerase

1JPM の概要

• エントリーDOI: 10.2210/pdb1jpm/pdb
• 関連するPDBエントリー: 1FHV 1JPD 1MUC
• 分子名称: L-Ala-D/L-Glu Epimerase, MAGNESIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: enolase superfamily, muconate lactonizing subgroup, alpha-beta barrel, isomerase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 158267.64

構造登録者

Gulick, A.M.,Schmidt, D.M.Z.,Gerlt, J.A.,Rayment, I. (登録日: 2001-08-02, 公開日: 2001-12-21, 最終更新日: 2024-02-07)

主引用文献

Gulick, A.M.,Schmidt, D.M.,Gerlt, J.A.,Rayment, I.
Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis.
Biochemistry, 40:15716-15724, 2001

PubMed Abstract: The members of the enolase superfamily catalyze different overall reactions, yet share a partial reaction that involves Mg(2+)-assisted enolization of the substrate carboxylate anion. The fate of the resulting enolate intermediate is determined by the active site of each enzyme. Several members of this superfamily have been structurally characterized to permit an understanding of the evolutionary strategy for using a common structural motif to catalyze different overall reactions. In the preceding paper, two new members of the superfamily were identified that catalyze the epimerization of the glutamate residue in L-Ala-D/L-Glu. These enzymes belong to the muconate lactonizing enzyme subgroup of the enolase superfamily, and their sequences are only 31% identical. The structure of YcjG, the epimerase from Escherichia coli, was determined by MAD phasing using both the SeMet-labeled protein and a heavy atom derivative. The structure of YkfB, the epimerase from Bacillus subtilis, was determined by molecular replacement using the muconate lactonizing enzyme as a search model. In this paper, we report the three-dimensional structures of these enzymes and compare them to the structure of o-succinylbenzoate synthase, another member of the muconate lactonizing enzyme subgroup.

PubMed: 11747448
DOI: 10.1021/bi011641p

実験手法

X-RAY DIFFRACTION (2.25 Å)