1JH6

Semi-reduced Cyclic Nucleotide Phosphodiesterase from Arabidopsis thaliana

1JH6 の概要

• エントリーDOI: 10.2210/pdb1jh6/pdb
• 関連するPDBエントリー: 1fsi 1jh7
• 分子名称: cyclic phosphodiesterase, SULFATE ION (3 entities in total)
• 機能のキーワード: adp-ribose 1'', 2''-cyclic phosphate, rna processing, 2', 3'-cyclic nucleotide phosphodiesterase, hydrolase
• 由来する生物種: Arabidopsis thaliana (thale cress)
• 細胞内の位置: Cytoplasm: O04147
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43404.79

構造登録者

Hofmann, A.,Grella, M.,Botos, I.,Filipowicz, W.,Wlodawer, A. (登録日: 2001-06-27, 公開日: 2002-02-06, 最終更新日: 2024-10-30)

主引用文献

Hofmann, A.,Grella, M.,Botos, I.,Filipowicz, W.,Wlodawer, A.
Crystal structures of the semireduced and inhibitor-bound forms of cyclic nucleotide phosphodiesterase from Arabidopsis thaliana.
J.Biol.Chem., 277:1419-1425, 2002

PubMed Abstract: The crystal structure of the semireduced form of cyclic nucleotide phosphodiesterase (CPDase) from Arabidopsis thaliana has been solved by molecular replacement and refined at the resolution of 1.8 A. We have previously reported the crystal structure of the native form of this enzyme, whose main target is ADP-ribose 1",2"-cyclic phosphate, a product of the tRNA splicing reaction. CPDase possesses six cysteine residues, four of which are involved in forming two intra-molecular disulfide bridges. One of these bridges, between Cys-104 and Cys-110, is opened in the semireduced CPDase, whereas the other remains intact. This change of the redox state leads to a conformational rearrangement in the loop covering the active site of the protein. While the native structure shows this partially disordered loop in a coil conformation, in the semireduced enzyme the N-terminal lobe of this loop winds up and elongates the preceding alpha-helix. The semireduced state of CPDase also enabled co-crystallization with a putative inhibitor of its enzymatic activity, 2',3'-cyclic uridine vanadate. The ligand is bound within the active site, and the mode of binding is in agreement with the previously proposed enzymatic mechanism. Selected biophysical properties of the oxidized and the semireduced CPDase are also discussed.

PubMed: 11694509
DOI: 10.1074/jbc.M107889200

実験手法

X-RAY DIFFRACTION (1.8 Å)