Crystal Structure of the Active Site Mutant Glu328Gln of Amylosucrase from Neisseria polysaccharea in Complex with the Natural Substrate Sucrose

Summary for 1JGI

Related PRD IDPRD_900003
Descriptoramylosucrase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose (3 entities in total)
Functional Keywordsactive site mutant glu328gln, sucrose complex, transferase
Biological sourceNeisseria polysaccharea
Cellular locationSecreted  Q9ZEU2
Total number of polymer chains1
Total molecular weight71930.41
Mirza, O.,Skov, L.K.,Gajhede, M. (deposition date: 2001-06-25, release date: 2001-10-31, Last modification date: 2020-07-29)
Primary citation
Mirza, O.,Skov, L.K.,Remaud-Simeon, M.,Potocki de Montalk, G.,Albenne, C.,Monsan, P.,Gajhede, M.
Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose.
Biochemistry, 40:9032-9039, 2001
PubMed: 11467966 (PDB entries with the same primary citation)
DOI: 10.1021/bi010706l
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliers8 0.2% 2.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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PDB entries from 2020-09-23