1JG5

CRYSTAL STRUCTURE OF RAT GTP CYCLOHYDROLASE I FEEDBACK REGULATORY PROTEIN, GFRP

Summary for 1JG5

• Entry DOI: 10.2210/pdb1jg5/pdb
• Descriptor: GTP CYCLOHYDROLASE I FEEDBACK REGULATORY PROTEIN, POTASSIUM ION (3 entities in total)
• Functional Keywords: alpha/beta structure, beta sheet, protein binding
• Biological source: Rattus norvegicus (Norway rat)
• Cellular location: Nucleus (By similarity): P70552
• Total number of polymer chains: 5
• Total formula weight: 47955.64

Authors

Bader, G.,Schiffmann, S.,Herrmann, A.,Fischer, M.,Gutlich, M.,Auerbach, G.,Ploom, T.,Bacher, A.,Huber, R.,Lemm, T. (deposition date: 2001-06-23, release date: 2001-10-10, Last modification date: 2024-03-13)

Primary citation

Bader, G.,Schiffmann, S.,Herrmann, A.,Fischer, M.,Gutlich, M.,Auerbach, G.,Ploom, T.,Bacher, A.,Huber, R.,Lemm, T.
Crystal structure of rat GTP cyclohydrolase I feedback regulatory protein, GFRP.
J.Mol.Biol., 312:1051-1057, 2001

PubMed Abstract: Tetrahydrobiopterin, the cofactor required for hydroxylation of aromatic amino acids regulates its own synthesis in mammals through feedback inhibition of GTP cyclohydrolase I. This mechanism is mediated by a regulatory subunit called GTP cyclohydrolase I feedback regulatory protein (GFRP). The 2.6 A resolution crystal structure of rat GFRP shows that the protein forms a pentamer. This indicates a model for the interaction of mammalian GTP cyclohydrolase I with its regulator, GFRP. Kinetic investigations of human GTP cyclohydrolase I in complex with rat and human GFRP showed similar regulatory effects of both GFRP proteins.

PubMed: 11580249
DOI: 10.1006/jmbi.2001.5011

Experimental method

X-RAY DIFFRACTION (2.6 Å)