1JF5
CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A
Summary for 1JF5
Entry DOI | 10.2210/pdb1jf5/pdb |
Related | 1BVZ 1JF6 |
Descriptor | ALPHA AMYLASE II, CALCIUM ION (2 entities in total) |
Functional Keywords | beta/alpha barrel, hydrolase |
Biological source | Thermoactinomyces vulgaris |
Total number of polymer chains | 2 |
Total formula weight | 135036.19 |
Authors | Ohtaki, A.,Kondo, S.,Shimura, Y.,Tonozuka, T.,Sakano, Y.,Kamitori, S. (deposition date: 2001-06-20, release date: 2002-05-22, Last modification date: 2024-05-29) |
Primary citation | Ohtaki, A.,Kondo, S.,Shimura, Y.,Tonozuka, T.,Sakano, Y.,Kamitori, S. Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structures of the mutant TVAIIs, F286A and F286Y, and kinetic analyses of the Phe286-replaced mutant TVAIIs CARBOHYDR.RES., 334:309-313, 2001 Cited by PubMed: 11527532DOI: 10.1016/S0008-6215(01)00190-2 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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