1JEY

Crystal Structure of the Ku heterodimer bound to DNA

1JEY の概要

• エントリーDOI: 10.2210/pdb1jey/pdb
• 関連するPDBエントリー: 1JEQ
• 分子名称: DNA (5'-D(*GP*TP*TP*TP*TP*TP*AP*GP*TP*TP*TP*AP*TP*TP*GP*GP*GP*CP*GP*CP*G)-3'), DNA (34-MER), Ku70, ... (5 entities in total)
• 機能のキーワード: double-strand dna break repair, non-homologous end-joining, protein-nucleic acid complex, alpha/beta domain, beta barrel, helical c-terminal arm, sap domain, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P12956 P13010
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 150967.46

構造登録者

Walker, J.R.,Corpina, R.A.,Goldberg, J. (登録日: 2001-06-19, 公開日: 2001-08-10, 最終更新日: 2023-08-16)

主引用文献

Walker, J.R.,Corpina, R.A.,Goldberg, J.
Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair.
Nature, 412:607-614, 2001

PubMed Abstract: The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle two full turns of DNA while encircling only the central 3-4 base pairs (bp). Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features seem well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation.

PubMed: 11493912
DOI: 10.1038/35088000

実験手法

X-RAY DIFFRACTION (2.5 Å)