1JCH
Crystal Structure of Colicin E3 in Complex with its Immunity Protein
Summary for 1JCH
| Entry DOI | 10.2210/pdb1jch/pdb |
| Related | 1E44 3EIP |
| Descriptor | COLICIN E3, COLICIN E3 IMMUNITY PROTEIN, CITRIC ACID, ... (5 entities in total) |
| Functional Keywords | translocation domain is a beta-jellyroll, the receptor-binding domain is a coiled coil, the rnase domain is a six-stranded antiparallel beta-sheet. the immunity protein is a four-stranded antiparallel beta sheet flanked by 3 helices on one side of the sheet, ribosome inhibitor, hydrolase |
| Biological source | Escherichia coli str. K12 substr. More |
| Total number of polymer chains | 4 |
| Total formula weight | 136782.02 |
| Authors | Soelaiman, S.,Jakes, K.,Wu, N.,Li, C.,Shoham, M. (deposition date: 2001-06-09, release date: 2001-11-30, Last modification date: 2024-02-07) |
| Primary citation | Soelaiman, S.,Jakes, K.,Wu, N.,Li, C.,Shoham, M. Crystal structure of colicin E3: implications for cell entry and ribosome inactivation. Mol.Cell, 8:1053-1062, 2001 Cited by PubMed Abstract: Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome. PubMed: 11741540DOI: 10.1016/S1097-2765(01)00396-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.02 Å) |
Structure validation
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