1JCH

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

1JCH の概要

• エントリーDOI: 10.2210/pdb1jch/pdb
• 関連するPDBエントリー: 1E44 3EIP
• 分子名称: COLICIN E3, COLICIN E3 IMMUNITY PROTEIN, CITRIC ACID, ... (5 entities in total)
• 機能のキーワード: translocation domain is a beta-jellyroll, the receptor-binding domain is a coiled coil, the rnase domain is a six-stranded antiparallel beta-sheet. the immunity protein is a four-stranded antiparallel beta sheet flanked by 3 helices on one side of the sheet, ribosome inhibitor, hydrolase
• 由来する生物種: Escherichia coli str. K12 substr.; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 136782.02

構造登録者

Soelaiman, S.,Jakes, K.,Wu, N.,Li, C.,Shoham, M. (登録日: 2001-06-09, 公開日: 2001-11-30, 最終更新日: 2024-02-07)

主引用文献

Soelaiman, S.,Jakes, K.,Wu, N.,Li, C.,Shoham, M.
Crystal structure of colicin E3: implications for cell entry and ribosome inactivation.
Mol.Cell, 8:1053-1062, 2001

PubMed Abstract: Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome.

PubMed: 11741540
DOI: 10.1016/S1097-2765(01)00396-3

実験手法

X-RAY DIFFRACTION (3.02 Å)