1JBB

Ubiquitin Conjugating Enzyme, Ubc13

1JBB の概要

• エントリーDOI: 10.2210/pdb1jbb/pdb
• 分子名称: ubiquitin conjugating enzyme E2-17.5 KDA (2 entities in total)
• 機能のキーワード: ubiquitin, ubiquitin-conjugating enzyme, e2, ligase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34979.97

構造登録者

VanDemark, A.P.,Hofmann, R.M.,Tsui, C.,Pickart, C.M.,Wolberger, C. (登録日: 2001-06-03, 公開日: 2001-06-20, 最終更新日: 2023-08-16)

主引用文献

VanDemark, A.P.,Hofmann, R.M.,Tsui, C.,Pickart, C.M.,Wolberger, C.
Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer.
Cell(Cambridge,Mass.), 105:711-720, 2001

PubMed Abstract: While the signaling properties of ubiquitin depend on the topology of polyubiquitin chains, little is known concerning the molecular basis of specificity in chain assembly and recognition. UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and I kappa B alpha kinase activation. The crystal structure of the Mms2/Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites for the two substrate ubiquitins. Mutations that destabilize the heterodimer interface confer a marked UV sensitivity, providing direct evidence that the intact heterodimer is necessary for DNA repair. Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63-linked polyubiquitin signals.

PubMed: 11440714
DOI: 10.1016/S0092-8674(01)00387-7

実験手法

X-RAY DIFFRACTION (2 Å)