1J4V

CYANOVIRIN-N

1J4V の概要

• エントリーDOI: 10.2210/pdb1j4v/pdb
• 関連するPDBエントリー: 2EZM 2EZN 3EZM
• 分子名称: CYANOVIRIN-N (1 entity in total)
• 機能のキーワード: cyanovirin-n, hiv-inactivating, domain-swapped dimer, dipolar couplings / conjoined rigid body-torsion angle dynamics, immune system
• 由来する生物種: Nostoc ellipsosporum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22044.18

構造登録者

Clore, G.M.,Bewley, C.A. (登録日: 2001-11-21, 公開日: 2002-03-06, 最終更新日: 2023-12-27)

主引用文献

Clore, G.M.,Bewley, C.A.
Using conjoined rigid body/torsion angle simulated annealing to determine the relative orientation of covalently linked protein domains from dipolar couplings.
J.Magn.Reson., 154:329-335, 2002

PubMed Abstract: A simple and robust method for determining the relative orientations of covalently linked protein domains using conjoined rigid body/torsion angle dynamics simulated annealing on the basis of residual dipolar couplings is presented. In this approach each domain is treated as a rigid body and the relevant degrees of conformational freedom are restricted to the backbone torsion angles (phi, psi) of the linker between the domains. By this means translational information afforded by the presence of an intact linker is preserved. We illustrate this approach using the domain-swapped dimer of the HIV-inactivating protein cyanovirin-N as an example.

PubMed: 11846592
DOI: 10.1006/jmre.2001.2489

実験手法

SOLUTION NMR