1J30

The crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic and thermoacidiphilic archaeon

Summary for 1J30

• Entry DOI: 10.2210/pdb1j30/pdb
• Descriptor: 144aa long hypothetical rubrerythrin, FE (III) ION, ZINC ION, ... (5 entities in total)
• Functional Keywords: rubrerythrin, sulerythrin, sulfolobus tokodaii strain 7, four-helix bundle, domain swapping, metal binding site plasticity, structural genomics, electron transport
• Biological source: Sulfolobus tokodaii
• Total number of polymer chains: 2
• Total formula weight: 32502.66

Authors

Fushinobu, S.,Shoun, H.,Wakagi, T. (deposition date: 2003-01-16, release date: 2003-10-14, Last modification date: 2023-10-25)

Primary citation

Fushinobu, S.,Shoun, H.,Wakagi, T.
The Crystal Structure of Sulerythrin, A Rubrerythrin-like Protein from A Strictly Aerobic Archaeon, Sulfolobus tokodaii strain 7, shows unexpected domain swapping
Biochemistry, 42:11707-11715, 2003

PubMed Abstract: Sulerythrin is the first rubrerythrin-like protein to be isolated from an aerobic organism, Sulfolobus tokodaii strain 7, and it lacks a C-terminal rubredoxin-like FeS(4) domain. The protein purified from Sulfolobus cells was crystallized, and the crystal structure was determined at 1.7 A resolution. The dimer of sulerythrin exhibited "domain-swapping" at the loop connecting alphaB and alphaC, hybrid four-helix bundles consisting of alphaA/B and alphaC/D being formed. The structure and atomic identity of the binuclear metal center were determined by means of anomalous scattering analysis. The site contained 1.0 mol of hexacoordinate Fe, 0.80-0.87 mol of tetracoordinate Zn, and 0.73-0.88 mol of putative O(2) per monomer. The metal ions were found at exchanged positions compared to those in the Fe/Zn-containing rubrerythrin from Desulfovibrio vulgaris. The results demonstrate that the binuclear metal center of rubrerythrin-like proteins is plastic in its ability to bind metal ions.

PubMed: 14529281
DOI: 10.1021/bi034220b

Experimental method

X-RAY DIFFRACTION (1.7 Å)