1IZN

Crystal Structure of Actin Filament Capping Protein CapZ

1IZN の概要

• エントリーDOI: 10.2210/pdb1izn/pdb
• 分子名称: CapZ alpha-1 subunit, CapZ beta-1 subunit, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: heterodimer, capping protein, actin filament barbed end capping, protein binding
• 由来する生物種: Gallus gallus (chicken); 詳細
• 細胞内の位置: Cytoplasm, myofibril, sarcomere, Z line: P13127; Cytoplasm, cytoskeleton (By similarity): P14315
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129058.50

構造登録者

Yamashita, A.,Maeda, K.,Maeda, Y. (登録日: 2002-10-10, 公開日: 2003-04-08, 最終更新日: 2023-12-27)

主引用文献

Yamashita, A.,Maeda, K.,Maeda, Y.
Crystal structure of CapZ: structural basis for actin filament barbed end capping
EMBO J., 22:1529-1538, 2003

PubMed Abstract: Capping protein, a heterodimeric protein composed of alpha and beta subunits, is a key cellular component regulating actin filament assembly and organization. It binds to the barbed ends of the filaments and works as a 'cap' by preventing the addition and loss of actin monomers at the end. Here we describe the crystal structure of the chicken sarcomeric capping protein CapZ at 2.1 A resolution. The structure shows a striking resemblance between the alpha and beta subunits, so that the entire molecule has a pseudo 2-fold rotational symmetry. CapZ has a pair of mobile extensions for actin binding, one of which also provides concomitant binding to another protein for the actin filament targeting. The mobile extensions probably form flexible links to the end of the actin filament with a pseudo 2(1) helical symmetry, enabling the docking of the two in a symmetry mismatch.

PubMed: 12660160
DOI: 10.1093/emboj/cdg167

実験手法

X-RAY DIFFRACTION (2.1 Å)