1ISK
3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES
Summary for 1ISK
| Entry DOI | 10.2210/pdb1isk/pdb |
| Descriptor | 3-OXO-DELTA5-STEROID ISOMERASE (1 entity in total) |
| Functional Keywords | isomerase, ksi, 3-ketosteroid |
| Biological source | Comamonas testosteroni |
| Total number of polymer chains | 2 |
| Total formula weight | 26824.20 |
| Authors | Wu, Z.R.,Ebrahimian, S.,Zawrotny, M.E.,Thornburg, L.D.,Perez-Alvarado, G.C.,Brothers, P.,Pollack, R.M.,Summers, M.F. (deposition date: 1997-03-12, release date: 1997-11-12, Last modification date: 2024-05-22) |
| Primary citation | Wu, Z.R.,Ebrahimian, S.,Zawrotny, M.E.,Thornburg, L.D.,Perez-Alvarado, G.C.,Brothers, P.,Pollack, R.M.,Summers, M.F. Solution structure of 3-oxo-delta5-steroid isomerase. Science, 276:415-418, 1997 Cited by PubMed Abstract: The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate. PubMed: 9103200DOI: 10.1126/science.276.5311.415 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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