1IPC

CRYSTAL STRUCTURE OF EUKARYOTIC INITIATION FACTOR 4E COMPLEXED WITH 7-METHYL GTP

Summary for 1IPC

• Entry DOI: 10.2210/pdb1ipc/pdb
• Related: 1IPB
• Descriptor: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E, 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE (3 entities in total)
• Functional Keywords: initiation factor, protein biosynthesis, rna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm, P-body : P06730
• Total number of polymer chains: 1
• Total formula weight: 25668.46

Authors

Tomoo, K.,Shen, X.,Okabe, K.,Nozoe, Y.,Fukuhara, S.,Morino, S.,Ishida, T.,Taniguchi, T.,Hasegawa, H.,Terashima, A.,Sasaki, M.,Katsuya, Y.,Kitamura, K.,Miyoshi, H.,Ishikawa, M.,Miura, K. (deposition date: 2001-05-08, release date: 2002-05-08, Last modification date: 2023-12-27)

Primary citation

Tomoo, K.,Shen, X.,Okabe, K.,Nozoe, Y.,Fukuhara, S.,Morino, S.,Ishida, T.,Taniguchi, T.,Hasegawa, H.,Terashima, A.,Sasaki, M.,Katsuya, Y.,Kitamura, K.,Miyoshi, H.,Ishikawa, M.,Miura, K.
Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region
BIOCHEM.J., 362:539-544, 2002

PubMed Abstract: The crystal structures of the full-length human eukaryotic initiation factor (eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine 5'-triphosphate (m(7)GTP) and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)] were determined at 2.0 A resolution (where 1 A=0.1 nm). The flexibility of the C-terminal loop region of eIF4E complexed with m(7)GTP was significantly reduced when complexed with m(7)GpppA, suggesting the importance of the second nucleotide in the mRNA cap structure for the biological function of eIF4E, especially the fixation and orientation of the C-terminal loop region, including the eIF4E phosphorylation residue. The present results provide the structural basis for the biological function of both N- and C-terminal mobile regions of eIF4E in translation initiation, especially the regulatory function through the switch-on/off of eIF4E-binding protein-eIF4E phosphorylation.

PubMed: 11879179
DOI: 10.1042/0264-6021:3620539

Experimental method

X-RAY DIFFRACTION (2 Å)