1IK3

LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID

Summary for 1IK3

• Entry DOI: 10.2210/pdb1ik3/pdb
• Descriptor: LIPOXYGENASE-3, FE (III) ION, 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID, ... (7 entities in total)
• Functional Keywords: oxidoreductase, purple lipoxygenase, fe(iii) complex, interm
• Biological source: Glycine max (soybean)
• Cellular location: Cytoplasm: P09186
• Total number of polymer chains: 1
• Total formula weight: 98224.62

Authors

Skrzypczak-Jankun, E.,Funk Jr., M.O. (deposition date: 2001-05-01, release date: 2001-11-14, Last modification date: 2024-12-25)

Primary citation

Skrzypczak-Jankun, E.,Bross, R.A.,Carroll, R.T.,Dunham, W.R.,Funk, M.O.
Three-dimensional structure of a purple lipoxygenase.
J.Am.Chem.Soc., 123:10814-10820, 2001

PubMed Abstract: Polyunsaturated fatty acid metabolism is governed primarily by two enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure of the metastable product-oxidized purple form of soybean lipoxygenase-3 was determined at 2.0 A resolution. The data reveal that the chromophore corresponds to an iron-peroxide complex, a potential intermediate in the catalyzed reaction. A significant alteration of the iron site accompanies the formation of the complex. The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity.

PubMed: 11686682
DOI: 10.1021/ja011759t

Experimental method

X-RAY DIFFRACTION (2 Å)