1IK1
Solution Structure of an RNA Hairpin from HRV-14
Summary for 1IK1
| Entry DOI | 10.2210/pdb1ik1/pdb |
| Descriptor | 5'-R(*GP*GP*UP*AP*CP*UP*AP*UP*GP*UP*AP*CP*CP*A)-3' (1 entity in total) |
| Functional Keywords | a form helix, triloop, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 4447.69 |
| Authors | Huang, H.,Alexandrov, A.,Chen, X.,Barnes III, T.W.,Zhang, H.,Dutta, K.,Pascal, S.M. (deposition date: 2001-05-01, release date: 2001-07-18, Last modification date: 2024-05-01) |
| Primary citation | Huang, H.,Alexandrov, A.,Chen, X.,Barnes III, T.W.,Zhang, H.,Dutta, K.,Pascal, S.M. Structure of an RNA hairpin from HRV-14. Biochemistry, 40:8055-8064, 2001 Cited by PubMed Abstract: The 5' noncoding region of the picornaviral genome begins with a cloverleaf which is required for viral replication, due at least in part to an interaction with the viral RNA polymerase as part of a fusion with the predominant viral protease. The necessary region of the cloverleaf has previously been narrowed to a highly conserved stem-loop. The solution structure of a 14-nucleotide RNA hairpin, which is part of the conserved stem-loop from human rhinovirus isotype 14, is presented here. The secondary structure of the hairpin is identical to predictions: a five base pair stem is bounded by a triloop with sequence UAU. However, the fold of the triloop is novel, with stacking of the second loop base onto the closing base pair of the stem, and deviations from A form geometry are introduced into the stem regions bordering the triloop, particularly on the 3' side. These deviations and the associated triloop structure could help to explain the distinct sequence conservation and mutational analysis data observed for the stem region of the hairpin, as compared to a second sequentially similar stem in the intact stem-loop. PubMed: 11434774DOI: 10.1021/bi010572b PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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