1IJE

Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex

1IJE の概要

• エントリーDOI: 10.2210/pdb1ije/pdb
• 関連するPDBエントリー: 1F60 1G7C
• 分子名称: elongation factor 1-alpha, elongation factor 1-beta, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: protein complex, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Cytoplasm : P02994
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60600.10

構造登録者

Andersen, G.R.,Valente, L.,Pedersen, L.,Kinzy, T.G.,Nyborg, J. (登録日: 2001-04-26, 公開日: 2001-05-09, 最終更新日: 2023-08-16)

主引用文献

Andersen, G.R.,Valente, L.,Pedersen, L.,Kinzy, T.G.,Nyborg, J.
Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex.
Nat.Struct.Biol., 8:531-534, 2001

PubMed Abstract: In the elongation cycle of protein biosynthesis, the nucleotide exchange factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved eEF1A-eEF1Balpha structure, we determined the structures of the eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha are consistent with in vivo data. The base, sugar and alpha-phosphate bind as in other known nucleotide G-protein complexes, whereas the beta- and gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that inserts into the Mg2+ binding site of eEF1A is lethal. This together with the structures emphasizes the essential role of Mg2+ in nucleotide exchange in the eEF1A-eEF1Balpha complex.

PubMed: 11373622
DOI: 10.1038/88598

実験手法

X-RAY DIFFRACTION (2.4 Å)