1IHD

Crystal Structure of Trigonal Form of D90E Mutant of Escherichia coli Asparaginase II

1IHD の概要

• エントリーDOI: 10.2210/pdb1ihd/pdb
• 関連するPDBエントリー: 1AGX 1DJO 1DJP 1HFJ 1HFK 1HO3 1WSA 3ECA 3PGA 4ECA 4PGA
• 分子名称: L-asparaginase II (2 entities in total)
• 機能のキーワード: l-asparaginase, leukemia, hydrolase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P00805
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69281.67

構造登録者

Borek, D.,Jaskolski, M. (登録日: 2001-04-19, 公開日: 2003-09-09, 最終更新日: 2024-10-16)

主引用文献

Borek, D.,Kozak, M.,Pei, J.,Jaskolski, M.
Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site.
Febs J., 281:4097-4111, 2014

PubMed Abstract: The periplasmic enzyme l-asparaginase type II from Escherichia coli (EcAII) converts l-asparagine to l-aspartate and ammonia. EcAII is an important drug in the treatment of childhood acute lymphoblastic leukemia, the most common malignancy in children. Leukemic blast cells lack the ability to synthesize l-asparagine and rely on other sources of l-asparagine for protein synthesis. EcAII injections deplete extracellular levels of l-asparagine, disrupting protein synthesis and inducing apoptosis in the malignant cells. The detailed mechanism of l-asparaginase catalytic action, the molecular mechanisms of its anticancer activity and the side effects associated with the treatment, including resistance to therapy, are not fully understood despite over 40 years of research. Here, we present X-ray structures of EcAII with an active site mutation, D90E, in three crystal forms. The region of the mutation is well ordered, allowing precise functional analysis of the consequences of the replacement of Asp90. In all three structures, the mutant protein exhibits an open conformation of the active site. In one of the structures, a zinc cation has been detected. The zinc cation is coordinated in a region of the protein that is implicated in the immunological response to EcAII treatment. A combined sequence-structure analysis of bacterial-type l-asparaginases reveals that the metal coordination may play a role in the response to asparaginase treatment. The observation of a zinc-binding site in antileukemic l-asparaginases provides new insight, with consequences for acute lymphoblastic leukemia therapy.

PubMed: 25040257
DOI: 10.1111/febs.12906

実験手法

X-RAY DIFFRACTION (2.65 Å)