1IHD
Crystal Structure of Trigonal Form of D90E Mutant of Escherichia coli Asparaginase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.255, 123.255, 83.874 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.650 |
| R-factor | 0.1686 |
| Rwork | 0.166 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Active dimer (AC) from native L-asparaginase II structure - PDB code: 3ECA |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.535 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.109 | 0.542 |
| Number of reflections | 21041 | |
| <I/σ(I)> | 11.2 | 2.2 |
| Completeness [%] | 97.3 | 96.4 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | sodium citrate, HEPES, PH 7.5, 292 K, VAPOR DIFFUSION, HANGING DROP |
