1IG9

Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase

Summary for 1IG9

• Entry DOI: 10.2210/pdb1ig9/pdb
• Related: 1CLQ 1WAJ
• Descriptor: 5'-D(*AP*CP*AP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3', 5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*(DOC))-3', DNA POLYMERASE, ... (6 entities in total)
• Functional Keywords: dna polymerase, catalytic complex, fidelity, two metal ion mechanism, transferase-dna complex, transferase/dna
• Biological source: Enterobacteria phage RB69
• Total number of polymer chains: 3
• Total formula weight: 115088.99

Authors

Franklin, M.C.,Wang, J.,Steitz, T.A. (deposition date: 2001-04-17, release date: 2001-06-11, Last modification date: 2023-08-16)

Primary citation

Franklin, M.C.,Wang, J.,Steitz, T.A.
Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase
Cell(Cambridge,Mass.), 105:657-667, 2001

PubMed Abstract: We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.

PubMed: 11389835
DOI: 10.1016/S0092-8674(01)00367-1

Experimental method

X-RAY DIFFRACTION (2.6 Å)