1IG7

Msx-1 Homeodomain/DNA Complex Structure

1IG7 の概要

• エントリーDOI: 10.2210/pdb1ig7/pdb
• 分子名称: 5'-D(*CP*AP*CP*TP*AP*AP*TP*TP*GP*AP*AP*GP*G)-3', 5'-D(P*TP*CP*CP*TP*TP*CP*AP*AP*TP*TP*AP*GP*TP*GP*AP*C)-3', Homeotic protein Msx-1, ... (4 entities in total)
• 機能のキーワード: helix-turn-helix, transcription-dna complex, transcription/dna
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P13297
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 15786.88

構造登録者

Hovde, S.,Abate-Shen, C.,Geiger, J.H. (登録日: 2001-04-17, 公開日: 2001-04-23, 最終更新日: 2024-04-03)

主引用文献

Hovde, S.,Abate-Shen, C.,Geiger, J.H.
Crystal structure of the Msx-1 homeodomain/DNA complex
Biochemistry, 40:12013-12021, 2001

PubMed Abstract: The Msx-1 homeodomain protein plays a crucial role in craniofacial, limb, and nervous system development. Homeodomain DNA-binding domains are comprised of 60 amino acids that show a high degree of evolutionary conservation. We have determined the structure of the Msx-1 homeodomain complexed to DNA at 2.2 A resolution. The structure has an unusually well-ordered N-terminal arm with a unique trajectory across the minor groove of the DNA. DNA specificity conferred by bases flanking the core TAAT sequence is explained by well ordered water-mediated interactions at Q50. Most interactions seen at the TAAT sequence are typical of the interactions seen in other homeodomain structures. Comparison of the Msx-1-HD structure to all other high resolution HD-DNA complex structures indicate a remarkably well-conserved sphere of hydration between the DNA and protein in these complexes.

PubMed: 11580277
DOI: 10.1021/bi0108148

実験手法

X-RAY DIFFRACTION (2.2 Å)