1IF2

X-RAY STRUCTURE OF LEISHMANIA MEXICANA TRIOSEPHOSPHATE ISOMERASE COMPLEXED WITH IPP

Summary for 1IF2

• Entry DOI: 10.2210/pdb1if2/pdb
• Descriptor: TRIOSEPHOSPHATE ISOMERASE, [2(FORMYL-HYDROXY-AMINO)-ETHYL]-PHOSPHONIC ACID (3 entities in total)
• Functional Keywords: tim barrel, transition state analogue, isomerase
• Biological source: Leishmania mexicana
• Cellular location: Cytoplasm: P48499
• Total number of polymer chains: 1
• Total formula weight: 27377.31

Authors

Kursula, I.,Partanen, S.,Lambeir, A.-M.,Antonov, D.M.,Augustyns, K.,Wierenga, R.K. (deposition date: 2001-04-12, release date: 2001-08-17, Last modification date: 2024-02-07)

Primary citation

Kursula, I.,Partanen, S.,Lambeir, A.M.,Antonov, D.M.,Augustyns, K.,Wierenga, R.K.
Structural determinants for ligand binding and catalysis of triosephosphate isomerase.
Eur.J.Biochem., 268:5189-5196, 2001

PubMed Abstract: The crystal structure of leishmania triosephosphate isomerase (TIM) complexed with 2-(N-formyl-N-hydroxy)-aminoethyl phosphonate (IPP) highlights the importance of Asn11 for binding and catalysis. IPP is an analogue of the substrate D-glyceraldehyde-3-phosphate, and it is observed to bind with its aldehyde oxygen in an oxyanion hole formed by ND2 of Asn11 and NE2 of His95. Comparison of the mode of binding of IPP and the transition state analogue phosphoglycolohydroxamate (PGH) suggests that the Glu167 side chain, as well as the triose part of the substrate, adopt different conformations as the catalysed reaction proceeds. Comparison of the TIM-IPP and the TIM-PGH structures with other liganded and unliganded structures also highlights the conformational flexibility of the ligand and the active site, as well as the conserved mode of ligand binding.

PubMed: 11589711
DOI: 10.1046/j.0014-2956.2001.02452.x

Experimental method

X-RAY DIFFRACTION (2 Å)