Summary for 1HYK
| Entry DOI | 10.2210/pdb1hyk/pdb |
| Related | 1qu8 |
| Descriptor | AGOUTI RELATED PROTEIN (1 entity in total) |
| Functional Keywords | cysteine rich; disulfide bond, ick, inhibitor cystine knot, melanocortin antagonists, agouti, agouti-related, agrp, endogenous antagonist, signaling protein |
| Total number of polymer chains | 1 |
| Total formula weight | 5207.16 |
| Authors | Bolin, K.A.,Anderson, D.J.,Trulson, J.A.,Thompson, D.A.,Wilken, J.,Kent, S.B.H.,Millhauser, G.L. (deposition date: 2001-01-19, release date: 2001-02-07, Last modification date: 2024-10-30) |
| Primary citation | Bolin, K.A.,Anderson, D.J.,Trulson, J.A.,Thompson, D.A.,Wilken, J.,Kent, S.B.,Gantz, I.,Millhauser, G.L. NMR structure of a minimized human agouti related protein prepared by total chemical synthesis. FEBS Lett., 451:125-131, 1999 Cited by PubMed Abstract: The structure of the chemically synthesized C-terminal region of the human agouti related protein (AGRP) was determined by 2D 1H NMR. Referred to as minimized agouti related protein, MARP is a 46 residue polypeptide containing 10 Cys residues involved in five disulfide bonds that retains the biological activity of full length AGRP. AGRP is a mammalian signaling molecule, involved in weight homeostasis, that causes adult onset obesity when overexpressed in mice. AGRP was originally identified by homology to the agouti protein, another potent signaling molecule involved in obesity disorders in mice. While AGRP's exact mechanism of action is unknown, it has been identified as a competitive antagonist of melanocortin receptors 3 and 4 (MC3r, MC4r), and MC4r in particular is implicated in the hypothalamic control of feeding behavior. Full length agouti and AGRP are only 25% homologous, however, their active C-terminal regions are approximately 40% homologous, with nine out of the 10 Cys residues spatially conserved. Until now, 3D structures have not been available for either agouti, AGRP or their C-terminal regions. The NMR structure of MARP reported here can be characterized as three major loops, with four of the five disulfide bridges at the base of the structure. Though its fold is well defined, no canonical secondary structure is identified. While previously reported structural models of the C-terminal region of AGRP were attempted based on Cys homology between AGRP and certain toxin proteins, we find that Cys spacing is not sufficient to correctly determine the 3D fold of the molecule. PubMed: 10371151DOI: 10.1016/S0014-5793(99)00553-0 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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