1HVU

HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 REVERSE TRANSCRIPTASE COMPLEXED WITH A 33-BASE NUCLEOTIDE RNA PSEUDOKNOT

1HVU の概要

• エントリーDOI: 10.2210/pdb1hvu/pdb
• 分子名称: RNA (33 NUCLEOTIDE RNA PSEUDOKNOT), PROTEIN (HIV-1 REVERSE TRANSCRIPTASE) (3 entities in total)
• 機能のキーワード: complex (nucleotidyltransferase-rna), hydrolase, aspartyl protease, endonuclease, transferase-rna complex, transferase/rna
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P03366 P03366
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 492023.45

構造登録者

Jaeger, J.,Restle, T.,Steitz, T.A. (登録日: 1998-06-30, 公開日: 1999-01-13, 最終更新日: 2023-08-02)

主引用文献

Jaeger, J.,Restle, T.,Steitz, T.A.
The structure of HIV-1 reverse transcriptase complexed with an RNA pseudoknot inhibitor.
EMBO J., 17:4535-4542, 1998

PubMed Abstract: Small RNA pseudoknots, selected to bind human immunodeficiency virus type 1 (HIV-1) reverse transcriptase tightly, are potent inhibitors of reverse transcriptase. The co-crystal structure of reverse transcriptase complexed with a 33 nucleotide RNA pseudoknot has been determined by fitting the ligand into a high quality, 4-fold averaged 4.8 A resolution electron density map. The RNA is kinked between stems S1 and S2, thereby optimizing its contacts with subunits of the heterodimer. Its binding site extends along the cleft that lies between the polymerase and RNase H active sites, partially overlaps with that observed for duplex DNA and presumably overlaps some portion of the tRNA site. Stem S2 and loop L1 stabilize the 'closed' conformation of the polymerase through extensive electrostatic interactions with several basic residues in helix I of the p66 thumb and in the p66 fingers domain. Presumably, this RNA ligand inhibits reverse transcriptase by binding to a site that partly overlaps the primer-template binding site.

PubMed: 9687519
DOI: 10.1093/emboj/17.15.4535

実験手法

X-RAY DIFFRACTION (4.75 Å)