1HNS

H-NS (DNA-BINDING DOMAIN)

Summary for 1HNS

• Entry DOI: 10.2210/pdb1hns/pdb
• Descriptor: H-NS (1 entity in total)
• Functional Keywords: histone-like protein h1, dna-binding protein, dna binding protein
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 5308.93

Authors

Shindo, H.,Iwaki, T.,Ieda, R.,Kurumizaka, H.,Ueguchi, C.,Mizuno, T.,Morikawa, S.,Nakamura, H.,Kuboniwa, H. (deposition date: 1995-04-06, release date: 1995-07-10, Last modification date: 2024-05-22)

Primary citation

Shindo, H.,Iwaki, T.,Ieda, R.,Kurumizaka, H.,Ueguchi, C.,Mizuno, T.,Morikawa, S.,Nakamura, H.,Kuboniwa, H.
Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli.
FEBS Lett., 360:125-131, 1995

PubMed Abstract: The three-dimensional structure of the C-terminal domain (47 residues) obtained from the hydrolysis of H-NS protein with bovine trypsin was determined by NMR measurements and distance geometry calculations. It is composed of an antiparallel beta-sheet, an alpha-helix and a 3(10)-helix which form a hydrophobic core, stabilizing the whole structure. This domain has been found to bind to DNA. Possible DNA binding sites are discussed on the basis of the solution structure of the C-terminal domain of H-NS.

PubMed: 7875316
DOI: 10.1016/0014-5793(95)00079-O

Experimental method

SOLUTION NMR