1HJK

ALKALINE PHOSPHATASE MUTANT H331Q

Summary for 1HJK

Entry DOI	10.2210/pdb1hjk/pdb
Descriptor	ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
Functional Keywords	alkaline phosphatase, hydrolase, phosphoric monoester, transferase(phospho, alcohol acceptor)
Biological source	Escherichia coli
Cellular location	Periplasm: P00634
Total number of polymer chains	2
Total formula weight	94831.09
Authors	Murphy, J.E.,Stec, B.,Ma, L.,Kantrowitz, E.R. (deposition date: 1997-05-30, release date: 1997-10-15, Last modification date: 2024-11-13)
Primary citation	Murphy, J.E.,Stec, B.,Ma, L.,Kantrowitz, E.R. Trapping and visualization of a covalent enzyme-phosphate intermediate. Nat.Struct.Biol., 4:618-622, 1997 Cited by PubMed Abstract: Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate. PubMed: 9253408 DOI: 10.1038/nsb0897-618 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.3 Å)

Structure validation