1HJI

BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX

Summary for 1HJI

• Entry DOI: 10.2210/pdb1hji/pdb
• Related: 1E4Z
• Descriptor: RNA (5-R(P*GP*CP*CP*CP*UP*GP*AP*AP*AP*AP*AP*GP*GP*GP*C)-3), NUN-PROTEIN (2 entities in total)
• Functional Keywords: bacteriophage hk022, termination, peptide-rna-complex, peptide-rna-recognition, protein/rna
• Biological source: BACTERIOPHAGE LAMBDA; More
• Total number of polymer chains: 2
• Total formula weight: 8021.65

Authors

Faber, C.,Schaerpf, M.,Becker, T.,Sticht, H.,Roesch, P. (deposition date: 2001-01-15, release date: 2002-01-29, Last modification date: 2024-05-15)

Primary citation

Faber, C.,Schaerpf, M.,Becker, T.,Sticht, H.,Roesch, P.
The Structure of the Coliphage Hk022 Nun Protein-Lambda-Phage Boxb RNA Complex. Implications for the Mechanism of Transcription Termination
J.Biol.Chem., 276:32064-, 2001

PubMed Abstract: Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.

PubMed: 11356847
DOI: 10.1074/JBC.M102975200

Experimental method

SOLUTION NMR