1HG3

Crystal structure of tetrameric TIM from Pyrococcus woesei.

1HG3 の概要

• エントリーDOI: 10.2210/pdb1hg3/pdb
• 分子名称: TRIOSEPHOSPHATE ISOMERASE, 3-PHOSPHONOPROPANOIC ACID (3 entities in total)
• 機能のキーワード: isomerase, thermostability, pyrococcus, triosephosphate isomerase, tetrameric
• 由来する生物種: PYROCOCCUS WOESEI
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 191021.99

構造登録者

Walden, H.,Bell, G.S.,Russell, R.J.M.,Siebers, B.,Hensel, R.,Taylor, G.L. (登録日: 2000-12-12, 公開日: 2001-03-07, 最終更新日: 2024-05-08)

主引用文献

Walden, H.,Bell, G.S.,Russell, R.J.M.,Siebers, B.,Hensel, R.,Taylor, G.L.
Tiny Tim: A Small, Tetrameric, Hyperthermostable Triosephosphate Isomerase
J.Mol.Biol., 306:745-, 2001

PubMed Abstract: Comparative structural studies on proteins derived from organisms with growth optima ranging from 15 to 100 degrees C are beginning to shed light on the mechanisms of protein thermoadaptation. One means of sustaining hyperthermostability is for proteins to exist in higher oligomeric forms than their mesophilic homologues. Triosephosphate isomerase (TIM) is one of the most studied enzymes, whose fold represents one of nature's most common protein architectures. Most TIMs are dimers of approximately 250 amino acid residues per monomer. Here, we report the 2.7 A resolution crystal structure of the extremely thermostable TIM from Pyrococcus woesei, a hyperthermophilic archaeon growing optimally at 100 degrees C, representing the first archaeal TIM structure. P. woesei TIM exists as a tetramer comprising monomers of only 228 amino acid residues. Structural comparisons with other less thermostable TIMs show that although the central beta-barrel is largely conserved, severe pruning of several helices and truncation of some loops give rise to a much more compact monomer in the small hyperthermophilic TIM. The classical TIM dimer formation is conserved in P. woesei TIM. The extreme thermostability of PwTIM appears to be achieved by the creation of a compact tetramer where two classical TIM dimers interact via an extensive hydrophobic interface. The tetramer is formed through largely hydrophobic interactions between some of the pruned helical regions. The equivalent helical regions in less thermostable dimeric TIMs represent regions of high average temperature factor. The PwTIM seems to have removed these regions of potential instability in the formation of the tetramer. This study of PwTIM provides further support for the role of higher oligomerisation states in extreme thermal stabilisation.

PubMed: 11243785
DOI: 10.1006/JMBI.2000.4433

実験手法

X-RAY DIFFRACTION (2.7 Å)