1HDY

THREE-DIMENSIONAL STRUCTURES OF THREE HUMAN ALCOHOL DEHYDROGENASE VARIANTS: CORRELATIONS WITH THEIR FUNCTIONAL DIFFERENCES

Summary for 1HDY

• Entry DOI: 10.2210/pdb1hdy/pdb
• Related: 1HDX 1HDY
• Descriptor: ALCOHOL DEHYDROGENASE, ZINC ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (6 entities in total)
• Functional Keywords: oxidoreductase(nad(a)-choh(d))
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P00325
• Total number of polymer chains: 2
• Total formula weight: 81520.35

Authors

Hurley, T.D.,Amzel, L.M. (deposition date: 1993-10-15, release date: 1994-01-31, Last modification date: 2024-02-07)

Primary citation

Hurley, T.D.,Bosron, W.F.,Stone, C.L.,Amzel, L.M.
Structures of three human beta alcohol dehydrogenase variants. Correlations with their functional differences.
J.Mol.Biol., 239:415-429, 1994

PubMed Abstract: The three-dimensional structures of three variants of human beta alcohol dehydrogenase have been determined to 2.5 A resolution. These three structures differ only in the amino acid at position 47 and the molecules occupying the alcohol binding site. Human beta 1 alcohol dehydrogenase has an Arg at position 47 and was crystallized in a complex with NAD(H) and cyclohexanol. A naturally occurring variant of beta 1 alcohol dehydrogenase, found in approximately 50% of the Asian population, possesses a His at position 47 (beta 2 or beta 47H) and was crystallized in a complex with NAD+ and the inhibitor 4-iodopyrazole. A site-directed mutant of beta 1 alcohol dehydrogenase in which a Gly is substituted for Arg47 (beta 47G) was crystallized in a complex with NAD+. By comparing both the common and unique features of these structures, it is clear that position 47 contributes significantly to the strength of protein-coenzyme interactions. The substitution of Arg47 by His produces an enzyme with a 100-fold lower affinity for coenzyme, but creates no large changes in the enzyme structure. The substitution of Arg47 by Gly produces an enzyme with coenzyme binding characteristics more similar to the wild-type enzyme than to the enzyme with His at position 47, but the structure of the Gly47 variant exhibits differences in and around the coenzyme binding site. These changes involve a rigid-body rotation of the catalytic domain towards the coenzyme domain by approximately 0.8 degrees and local rearrangements of amino acid side-chains, such as a 1.0 A movement of Lys228, relative to the beta 1 enzyme. These structural alterations may compensate for the loss of coenzyme interactions contributed by Arg47 and can explain the high affinity of the Gly47 variant for coenzyme.

PubMed: 8201622
DOI: 10.1006/jmbi.1994.1382

Experimental method

X-RAY DIFFRACTION (2.5 Å)