1HCJ

Photoproduct of the wild-type Aequorea victoria Green Fluorescent Protein

1HCJ の概要

• エントリーDOI: 10.2210/pdb1hcj/pdb
• 関連するPDBエントリー: 1B9C 1BFP 1C4F 1EMB 1EMC 1EME 1EMF 1EMG 1EMK 1EML 1EMM 1F09 1F0B 1YFP 2EMD 2EMN 2EMO
• 分子名称: GREEN FLUORESCENT PROTEIN (2 entities in total)
• 機能のキーワード: luminescent protein, fluorescent protein, beta-barrel, bioluminescence, luminescence
• 由来する生物種: AEQUOREA VICTORIA
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 107549.38

構造登録者

Van Thor, J.J.,Gensch, T.,Hellingwerf, K.J.,Johnson, L. (登録日: 2001-05-04, 公開日: 2001-12-07, 最終更新日: 2023-12-13)

主引用文献

Van Thor, J.J.,Gensch, T.,Hellingwerf, K.J.,Johnson, L.
Phototransformation of Green Fluorescent Protein with Uv and Visible Light Leads to Decarboxylation of Glutamate 222
Nat.Struct.Biol., 9:37-, 2002

PubMed Abstract: Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.

PubMed: 11740505
DOI: 10.1038/NSB739

実験手法

X-RAY DIFFRACTION (1.8 Å)