1HBX

Ternary Complex of SAP-1 and SRF with specific SRE DNA

Summary for 1HBX

• Entry DOI: 10.2210/pdb1hbx/pdb
• Related: 1BC7 1BC8 1SRS
• Descriptor: SERUM RESPONSE FACTOR, 5'-D(*GP*AP*TP*GP*GP*CP*CP*TP*AP*AP*TP*TP*AP* GP*GP*AP*CP*TP*TP*CP*CP*GP*GP*TP*G)-3', ETS-DOMAIN PROTEIN ELK-4, ... (4 entities in total)
• Functional Keywords: gene regulation, transcription complex, serum response factor, ternary complex factor
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Nucleus: P11831 P28324
• Total number of polymer chains: 10
• Total formula weight: 109097.94

Authors

Hassler, M.,Richmond, T.J. (deposition date: 2001-04-20, release date: 2001-06-27, Last modification date: 2023-12-13)

Primary citation

Hassler, M.,Richmond, T.J.
The B-Box Dominates Sap-1/Srf Interactions in the Structure of the Ternary Complex
Embo J., 20:3018-, 2001

PubMed Abstract: The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.

PubMed: 11406578
DOI: 10.1093/EMBOJ/20.12.3018

Experimental method

X-RAY DIFFRACTION (3.15 Å)