1H9H

COMPLEX OF EETI-II WITH PORCINE TRYPSIN

1H9H の概要

• エントリーDOI: 10.2210/pdb1h9h/pdb
• 関連するPDBエントリー: 1AKS 1AN1 1AVW 1AVX 1C9P 1D3O 1DF2 1EJA 1EPT 1EWU 1FMG 1FN6 1FNI 1H9H 1LDT 1LT2 1MCT 1QQU 1R0T 1S5S 1S6F 1S6H 1S81 1S82 1S83 1S84 1S85 1TFX
• 分子名称: TRYPSIN, TRYPSIN INHIBITOR II, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase inhibitor, complex (serine protease-inhibitor), trypsin, squash inhibitor, cystine knot, hydrolase
• 由来する生物種: SUS SCROFA (PIG); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27436.07

構造登録者

Kraetzner, R.,Wentzel, A.,Kolmar, H.,Uson, I. (登録日: 2001-03-12, 公開日: 2004-07-26, 最終更新日: 2024-11-06)

主引用文献

Kraetzner, R.,Debreczeni, J.E.,Pape, T.,Schneider, T.R.,Wentzel, A.,Kolmar, H.,Sheldrick, G.M.,Uson, I.
Structure of Ecballium Elaterium Trypsin Inhibitor II (Eeti-II): A Rigid Molecular Scaffold
Acta Crystallogr.,Sect.D, 61:1255-, 2005

PubMed Abstract: The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.

PubMed: 16131759
DOI: 10.1107/S0907444905021207

実験手法

X-RAY DIFFRACTION (1.5 Å)