1H8X

Domain-swapped Dimer of a Human Pancreatic Ribonuclease Variant

Summary for 1H8X

• Entry DOI: 10.2210/pdb1h8x/pdb
• Related: 1E21
• Descriptor: RIBONUCLEASE 1 (2 entities in total)
• Functional Keywords: hydrolase, ribonuclease, rnase, human pancreatic ribonuclease, domain- swapped dimer
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Secreted: P07998
• Total number of polymer chains: 2
• Total formula weight: 28908.34

Authors

Canals, A.,Pous, J.,Guasch, A.,Benito, A.,Ribo, M.,Vilanova, M.,Coll, M. (deposition date: 2001-02-16, release date: 2002-02-14, Last modification date: 2024-11-06)

Primary citation

Canals, A.,Pous, J.,Guasch, A.,Benito, A.,Ribo, M.,Vilanova, M.,Coll, M.
The Structure of an Engineered Domain-Swapped Ribonuclease Dimer and its Implications for the Evolution of Proteins Toward Oligomerization
Structure, 9:967-, 2001

PubMed Abstract: Domain swapping has been proposed as a mechanism that explains the evolution from monomeric to oligomeric proteins. Bovine and human pancreatic ribonucleases are monomers with no biological properties other than their RNA cleavage ability. In contrast, the closely related bovine seminal ribonuclease is a natural domain-swapped dimer that has special biological properties, such as cytotoxicity to tumour cells. Several recombinant ribonuclease variants are domain-swapped dimers, but a structure of this kind has not yet been reported for the human enzyme.

PubMed: 11591351
DOI: 10.1016/S0969-2126(01)00659-1

Experimental method

X-RAY DIFFRACTION (2 Å)