1H59

Complex of IGFBP-5 with IGF-I

1H59 の概要

• エントリーDOI: 10.2210/pdb1h59/pdb
• 関連するPDBエントリー: 2GF1 3GF1
• 分子名称: INSULIN-LIKE GROWTH FACTOR IA, INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 5 (3 entities in total)
• 機能のキーワード: insulin, insulin-like growth factor, igf binding protein
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13715.67

構造登録者

Zeslawski, W.,Beisel, H.G.,Kamionka, M.,Kalus, W.,Engh, R.A.,Huber, R.,Holak, T.A. (登録日: 2001-05-21, 公開日: 2002-05-16, 最終更新日: 2024-11-06)

主引用文献

Zesaawski, W.,Beisel, H.G.,Kamionka, M.,Kalus, W.,Engh, R.A.,Huber, R.,Lang, K.,Holak, T.A.
The Interaction of Insulin-Like Growth Factor-I with the N-Terminal Domain of Igfbp-5
Embo J., 20:3638-, 2001

PubMed Abstract: Insulin-like growth factors (IGFs) are key regulators of cell proliferation, differentiation and transformation, and are thus pivotal in cancer, especially breast, prostate and colon neoplasms. They are also important in many neurological and bone disorders. Their potent mitogenic and anti-apoptotic actions depend primarily on their availability to bind to the cell surface IGF-I receptor. In circulation and interstitial fluids, IGFs are largely unavailable as they are tightly associated with IGF-binding proteins (IGFBPs) and are released after IGFBP proteolysis. Here we report the 2.1 A crystal structure of the complex of IGF-I bound to the N-terminal IGF-binding domain of IGFBP-5 (mini-IGFBP-5), a prototype interaction for all N-terminal domains of the IGFBP family. The principal interactions in the complex comprise interlaced hydrophobic side chains that protrude from both IGF-I and the IGFBP-5 fragment and a surrounding network of polar interactions. A solvent-exposed hydrophobic patch is located on the IGF-I pole opposite to the mini-IGFBP-5 binding region and marks the IGF-I receptor binding site.

PubMed: 11447105
DOI: 10.1093/EMBOJ/20.14.3638

実験手法

X-RAY DIFFRACTION (2.1 Å)