1H59

Complex of IGFBP-5 with IGF-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	278
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 3
Unit cell lengths	74.385, 74.385, 74.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.200 - 2.100
R-factor	0.218
Rwork	0.218
R-free	0.26200
Structure solution method	SIR
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.200	2.230
High resolution limit [Å]	2.100	2.110
Rmerge	0.082	0.448
Total number of observations	45345 *
Number of reflections	8035
Completeness [%]	99.3 *	96.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	4 *	pH 5.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Jeffamine M-600	10 (%)
2	1	1	sodium citrate	0.1 (M)
3	1	1		0.01 (M)	pH5.6