1H34

Crystal structure of lima bean trypsin inhibitor

1H34 の概要

• エントリーDOI: 10.2210/pdb1h34/pdb
• 分子名称: BOWMAN-BIRK TYPE PROTEINASE INHIBITOR (2 entities in total)
• 機能のキーワード: inhibitor, bowman-birk-type proteinase inhibitor, serine protease inhibitor
• 由来する生物種: PHASEOLUS LUNATUS (LIMA BEAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9043.96

構造登録者

Debreczeni, J.E.,Bunkoczi, G.,Girmann, B.,Sheldrick, G.M. (登録日: 2002-08-21, 公開日: 2003-02-06, 最終更新日: 2019-07-24)

主引用文献

Debreczeni, J.E.,Bunkoczi, G.,Girmann, B.,Sheldrick, G.M.
In-House Phase Determination of the Lima Bean Trypsin Inhibitor: A Low-Resolution Sulfur-Sad Case
Acta Crystallogr.,Sect.D, 59:393-, 2003

PubMed Abstract: SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.

PubMed: 12554963
DOI: 10.1107/S0907444902020917

実験手法

X-RAY DIFFRACTION (2.04 Å)