1H21

A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774

「1DDC」から置き換えられました

1H21 の概要

• エントリーDOI: 10.2210/pdb1h21/pdb
• 関連するPDBエントリー: 1DDC
• 分子名称: SPLIT-SORET CYTOCHROME C, HEME C (3 entities in total)
• 機能のキーワード: cytochrome, dimeric di-heme cytochrome, stacked heme arrangement, novel fold, novel iron-sulfur centre, electron transport, sulfate respiration
• 由来する生物種: DESULFOVIBRIO DESULFURICANS
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 112705.90

構造登録者

Abreu, I.A.,Lourenco, A.I.,Xavier, A.V.,Legall, J.,Coelho, A.V.,Matias, P.M.,Pinto, D.M.,Carrondo, M.A.,Teixeira, M.,Saraiva, L.M. (登録日: 2002-07-30, 公開日: 2003-02-20, 最終更新日: 2024-11-20)

主引用文献

Abreu, I.A.,Lourenco, A.I.,Xavier, A.V.,Legall, J.,Coelho, A.V.,Matias, P.M.,Pinto, D.M.,Armenia Carrondo, M.,Teixeira, M.,Saraiva, L.M.
A Novel Iron Centre in the Split-Soret Cytochrome C from Desulfovibrio Desulfuricans Atcc 27774
J.Biol.Inorg.Chem., 8:360-, 2003

PubMed Abstract: The facultative sulfate/nitrate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 harbours a split-Soret cytochrome c. This cytochrome is a homodimeric protein, having two bis-histidinyl c-type haems per monomer. It has an unique architecture at the haem domain: each haem has one of the coordinating histidines provided by the other monomer, and in each monomer the haems are parallel to each other, almost in van der Waals contact. This work reports the cloning and sequencing of the gene encoding for this cytochrome and shows, by transcriptional analysis, that it is more expressed in nitrate-grown cells than in sulfate-grown ones. In addition, the gene-deduced amino acid sequence revealed two new cysteine residues that could be involved in the binding of a non-haem iron centre. Indeed, the presence of a novel type of an iron-sulfur centre (possibly of the [2Fe-2S] type) was demonstrated by EPR spectroscopy, and putative models for its localization and structure in the cytochrome molecule are proposed on the basis of the so-far-known 3D crystallographic structure of the aerobically purified split-Soret cytochrome, which lacks this centre.

PubMed: 12589573
DOI: 10.1007/S00775-002-0426-3

実験手法

X-RAY DIFFRACTION (2.5 Å)