1GZ0

23S RIBOSOMAL RNA G2251 2'O-METHYLTRANSFERASE RLMB

Summary for 1GZ0

• Entry DOI: 10.2210/pdb1gz0/pdb
• Descriptor: HYPOTHETICAL TRNA/RRNA METHYLTRANSFERASE YJFH (2 entities in total)
• Functional Keywords: transferase, methyltransferase, 2'o-methyltransferase, knot, montreal- kingston bacterial structural genomics initiative, bsgi, structural genomics
• Biological source: ESCHERICHIA COLI
• Total number of polymer chains: 8
• Total formula weight: 223559.05

Authors

Michel, G.,Cygler, M. (deposition date: 2002-05-03, release date: 2002-10-17, Last modification date: 2024-11-20)

Primary citation

Michel, G.,Sauve, V.,Larocque, R.,Li, Y.,Matte, A.,Cygler, M.
The Structure of the Rlmb 23S Rrna Methyltransferase Reveals a New Methyltransferase Fold with a Unique Knot
Structure, 10:1303-, 2002

PubMed Abstract: In Escherichia coli, RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. The crystal structure of this 2'O-methyltransferase has been determined at 2.5 A resolution. RlmB consists of an N-terminal domain connected by a flexible extended linker to a catalytic C-terminal domain and forms a dimer in solution. The C-terminal domain displays a divergent methyltransferase fold with a unique knotted region, and lacks the classic AdoMet binding site features. The N-terminal domain is similar to ribosomal proteins L7 and L30, suggesting a role in 23S rRNA recognition. The conserved residues in this novel family of 2'O-methyltransferases cluster in the knotted region, suggesting the location of the catalytic and AdoMet binding sites.

PubMed: 12377117
DOI: 10.1016/S0969-2126(02)00852-3

Experimental method

X-RAY DIFFRACTION (2.5 Å)