1GW6

STRUCTURE OF LEUKOTRIENE A4 HYDROLASE D375N MUTANT

1GW6 の概要

• エントリーDOI: 10.2210/pdb1gw6/pdb
• 関連するPDBエントリー: 1HS6
• 分子名称: LEUKOTRIENE A-4 HYDROLASE, 2-(3-AMINO-2-HYDROXY-4-PHENYL-BUTYRYLAMINO)-4-METHYL-PENTANOIC ACID, ACETATE ION, ... (7 entities in total)
• 機能のキーワード: hydrolase, mutagenesis studies, alpha-beta protein
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: P09960
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69906.74

構造登録者

Rudberg, P.C.,Tholander, F.,Thunnissen, M.M.G.M.,Samuelsson, B.,Haeggstrom, J.Z. (登録日: 2002-03-07, 公開日: 2003-03-06, 最終更新日: 2023-12-13)

主引用文献

Rudberg, P.C.,Tholander, F.,Thunnissen, M.M.G.M.,Samuelsson, B.,Haeggstrom, J.Z.
Leukotriene A4 Hydrolase: Selective Abrogation of Leukotriene B4 Formation by Mutation of Aspartic Acid 375
Proc.Natl.Acad.Sci.USA, 99:4215-, 2002

PubMed Abstract: Leukotriene A4 (LTA4, 5S-trans-5,6-oxido-7,9-trans-11,14-cis-eicosatetraenoic acid) hydrolase (LTA4H)/aminopeptidase is a bifunctional zinc metalloenzyme that catalyzes the final and rate-limiting step in the biosynthesis of leukotriene B4 (LTB4, 5S,12R-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid), a classical chemoattractant and immune modulating lipid mediator. Two chemical features are key to the bioactivity of LTB4, namely, the chirality of the 12R-hydroxyl group and the cis-trans-trans geometry of the conjugated triene structure. From the crystal structure of LTA4H, a hydrophilic patch composed of Gln-134, Tyr-267, and Asp-375 was identified in a narrow and otherwise hydrophobic pocket, believed to bind LTA4. In addition, Asp-375 belongs to peptide K21, a previously characterized 21-residue active site-peptide to which LTA4 binds during suicide inactivation. In the present report we used site-directed mutagenesis and x-ray crystallography to show that Asp-375, but none of the other candidate residues, is specifically required for the epoxide hydrolase activity of LTA4H. Thus, mutation of Asp-375 leads to a selective loss of the enzyme's ability to generate LTB4 whereas the aminopeptidase activity is preserved. We propose that Asp-375, possibly assisted by Gln-134, acts as a critical determinant for the stereoselective introduction of the 12R-hydroxyl group and thus the biological activity of LTB4.

PubMed: 11917124
DOI: 10.1073/PNAS.072090099

実験手法

X-RAY DIFFRACTION (2.2 Å)