1GV4

Murine apoptosis-inducing factor (AIF)

1GV4 の概要

• エントリーDOI: 10.2210/pdb1gv4/pdb
• 分子名称: PROGRAMED CELL DEATH PROTEIN 8, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: oxidoreductase, flavoprotein, fad, nuclear protein, apoptosi
• 由来する生物種: MUS MUSCULUS
• 細胞内の位置: Mitochondrion intermembrane space: Q9Z0X1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116713.95

構造登録者

Mate, M.J.,Alzari, P.M. (登録日: 2002-02-05, 公開日: 2002-04-26, 最終更新日: 2023-12-13)

主引用文献

Mate, M.J.,Ortiz-Lombardia, M.,Alzari, P.M.,Boitel, B.,Haouz, A.,Tello, D.,Susin, S.A.,Penninger, J.,Kroemer, G.,Alzari, P.M.
The Crystal Structure of the Mouse Apoptosis-Inducing Factor Aif
Nat.Struct.Biol., 9:442-, 2002

PubMed Abstract: Mitochondria play a key role in apoptosis due to their capacity to release potentially lethal proteins. One of these latent death factors is cytochrome c, which can stimulate the proteolytic activation of caspase zymogens. Another important protein is apoptosis-inducing factor (AIF), a flavoprotein that can stimulate a caspase-independent cell-death pathway required for early embryonic morphogenesis. Here, we report the crystal structure of mouse AIF at 2.0 A. Its active site structure and redox properties suggest that AIF functions as an electron transferase with a mechanism similar to that of the bacterial ferredoxin reductases, its closest evolutionary homologs. However, AIF structurally differs from these proteins in some essential features, including a long insertion in a C-terminal beta-hairpin loop, which may be related to its apoptogenic functions.

PubMed: 11967568
DOI: 10.1038/NSB793

実験手法

X-RAY DIFFRACTION (2 Å)