1GQ8

Pectin methylesterase from Carrot

1GQ8 の概要

• エントリーDOI: 10.2210/pdb1gq8/pdb
• 分子名称: PECTINESTERASE, CACODYLATE ION (3 entities in total)
• 機能のキーワード: hydrolase, carboxylic ester hydrolase
• 由来する生物種: DAUCUS CAROTA (CARROT)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34500.22

構造登録者

Johansson, K.,El-Ahmad, M.,Friemann, R.,Jornvall, H.,Markovic, O.,Eklund, H. (登録日: 2001-11-20, 公開日: 2002-04-18, 最終更新日: 2024-10-23)

主引用文献

Johansson, K.,El-Ahmad, M.,Friemann, R.,Jornvall, H.,Markovic, O.,Eklund, H.
Crystal Structure of Plant Pectin Methylesterase
FEBS Lett., 514:243-, 2002

PubMed Abstract: Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The beta-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state.

PubMed: 11943159
DOI: 10.1016/S0014-5793(02)02372-4

実験手法

X-RAY DIFFRACTION (1.75 Å)