1GQ2

Malic Enzyme from Pigeon Liver

Summary for 1GQ2

• Entry DOI: 10.2210/pdb1gq2/pdb
• Descriptor: MALIC ENZYME, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, OXALATE ION, ... (7 entities in total)
• Functional Keywords: oxidoreductase, malic enzyme, pigeon liver, nadp-dependent, nad-nadp selectivity, decarboxylase, malate, mn2+
• Biological source: COLUMBA LIVIA (DOMESTIC PIGEON)
• Cellular location: Cytoplasm: P40927
• Total number of polymer chains: 16
• Total formula weight: 1016763.75

Authors

Yang, Z.,Zhang, H.,Liang, T. (deposition date: 2001-11-19, release date: 2002-05-23, Last modification date: 2024-10-16)

Primary citation

Yang, Z.,Zhang, H.,Huang, H.-C.,Kuo, C.-C.,Tsai, L.-C.,Yuan, H.S.,Chou, W.-Y.,Chang, G.-G.,Tong, L.
Structural Studies of the Pigeon Cytosolic Nadp+ -Dependent Malic Enzyme
Protein Sci., 11:332-, 2002

PubMed Abstract: Malic enzymes are widely distributed in nature, and have important biological functions. They catalyze the oxidative decarboxylation of malate to produce pyruvate and CO(2) in the presence of divalent cations (Mg(2+), Mn(2+)). Most malic enzymes have a clear selectivity for the dinucleotide cofactor, being able to use either NAD(+) or NADP(+), but not both. Structural studies of the human mitochondrial NAD(+)-dependent malic enzyme established that malic enzymes belong to a new class of oxidative decarboxylases. Here we report the crystal structure of the pigeon cytosolic NADP(+)-dependent malic enzyme, in a closed form, in a quaternary complex with NADP(+), Mn(2+), and oxalate. This represents the first structural information on an NADP(+)-dependent malic enzyme. Despite the sequence conservation, there are large differences in several regions of the pigeon enzyme structure compared to the human enzyme. One region of such differences is at the binding site for the 2'-phosphate group of the NADP(+) cofactor, which helps define the cofactor selectivity of the enzymes. Specifically, the structural information suggests Lys362 may have an important role in the NADP(+) selectivity of the pigeon enzyme, confirming our earlier kinetic observations on the K362A mutant. Our structural studies also revealed differences in the organization of the tetramer between the pigeon and the human enzymes, although the pigeon enzyme still obeys 222 symmetry.

PubMed: 11790843
DOI: 10.1110/PS.38002

Experimental method

X-RAY DIFFRACTION (2.5 Å)