1GED
A positive charge route for the access of nadh to heme formed in the distal heme pocket of cytochrome p450nor
Summary for 1GED
| Entry DOI | 10.2210/pdb1ged/pdb |
| Descriptor | CYTOCHROME P450 55A1, BROMIDE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total) |
| Functional Keywords | cytochrome p450nor, nitric oxide reductase, oxidoreductase |
| Biological source | Fusarium oxysporum |
| Total number of polymer chains | 1 |
| Total formula weight | 45196.99 |
| Authors | Kudo, T.,Takaya, N.,Park, S.-Y.,Shiro, Y.,Shoun, H. (deposition date: 2000-11-02, release date: 2000-11-22, Last modification date: 2023-10-25) |
| Primary citation | Kudo, T.,Takaya, N.,Park, S.-Y.,Shiro, Y.,Shoun, H. A positively charged cluster formed in the heme-distal pocket of cytochrome P450nor is essential for interaction with NADH J.Biol.Chem., 276:5020-5026, 2001 Cited by PubMed Abstract: Arg and Lys residues are concentrated on the distal side of cytochrome P450nor (P450nor) to form a positively charged cluster facing from the outside to the inside of the distal heme pocket. We constructed mutant proteins in which the Arg and Lys residues were replaced with Glu, Gln, or Ala. The results showed that this cluster plays crucial roles in NADH interaction. We also showed that some anions such as bromide (Br(-)) perturbed the heme environment along with the reduction step in P450nor-catalyzed reactions, which was similar to the effects caused by the mutations. We determined by x-ray crystallography that a Br(-), termed an anion hole, occupies a key region neighboring heme, which is the terminus of the positively charged cluster and the terminus of the hydrogen bond network that acts as a proton delivery system. A comparison of the predicted mechanisms between the perturbations caused by Br(-) and the mutations suggested that Arg(174) and Arg(64) play a crucial role in binding NADH to the protein. These results indicated that the positively charged cluster is the unique structure of P450nor that responds to direct interaction with NADH. PubMed: 11076941DOI: 10.1074/jbc.M007244200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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