1G99

AN ANCIENT ENZYME: ACETATE KINASE FROM METHANOSARCINA THERMOPHILA

Summary for 1G99

• Entry DOI: 10.2210/pdb1g99/pdb
• Descriptor: ACETATE KINASE, SULFATE ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: alpha/beta; askha (acetate and sugar kinases, hsc70, actin) superfamily; conserved epsilon conformation; two similar domains, transferase
• Biological source: Methanosarcina thermophila
• Cellular location: Cytoplasm : P38502
• Total number of polymer chains: 2
• Total formula weight: 89834.50

Authors

Buss, K.A.,Cooper, D.R.,Ingram-Smith, C.,Ferry, J.G.,Sanders, D.A.,Hasson, M.S. (deposition date: 2000-11-22, release date: 2000-12-27, Last modification date: 2024-02-07)

Primary citation

Buss, K.A.,Cooper, D.R.,Ingram-Smith, C.,Ferry, J.G.,Sanders, D.A.,Hasson, M.S.
Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases.
J.Bacteriol., 183:680-686, 2001

PubMed Abstract: Acetate kinase, an enzyme widely distributed in the Bacteria and Archaea domains, catalyzes the phosphorylation of acetate. We have determined the three-dimensional structure of Methanosarcina thermophila acetate kinase bound to ADP through crystallography. As we previously predicted, acetate kinase contains a core fold that is topologically identical to that of the ADP-binding domains of glycerol kinase, hexokinase, the 70-kDa heat shock cognate (Hsc70), and actin. Numerous charged active-site residues are conserved within acetate kinases, but few are conserved within the phosphotransferase superfamily. The identity of the points of insertion of polypeptide segments into the core fold of the superfamily members indicates that the insertions existed in the common ancestor of the phosphotransferases. Another remarkable shared feature is the unusual, epsilon conformation of the residue that directly precedes a conserved glycine residue (Gly-331 in acetate kinase) that binds the alpha-phosphate of ADP. Structural, biochemical, and geochemical considerations indicate that an acetate kinase may be the ancestral enzyme of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of phosphotransferases.

PubMed: 11133963
DOI: 10.1128/JB.183.2.680-686.2001

Experimental method

X-RAY DIFFRACTION (2.5 Å)