1G47

1ST LIM DOMAIN OF PINCH PROTEIN

Summary for 1G47

• Entry DOI: 10.2210/pdb1g47/pdb
• NMR Information: BMRB: 4884
• Descriptor: PINCH PROTEIN, ZINC ION (2 entities in total)
• Functional Keywords: lim domain; zn finger, cell adhesion
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 8982.89

Authors

Velyvis, A.,Yang, Y.,Wu, C.,Qin, J. (deposition date: 2000-10-26, release date: 2001-02-21, Last modification date: 2024-05-22)

Primary citation

Velyvis, A.,Yang, Y.,Wu, C.,Qin, J.
Solution structure of the focal adhesion adaptor PINCH LIM1 domain and characterization of its interaction with the integrin-linked kinase ankyrin repeat domain.
J.Biol.Chem., 276:4932-4939, 2001

PubMed Abstract: PINCH is a recently identified adaptor protein that comprises an array of five LIM domains. PINCH functions through LIM-mediated protein-protein interactions that are involved in cell adhesion, growth, and differentiation. The LIM1 domain of PINCH interacts with integrin-linked kinase (ILK), thereby mediating focal adhesions via a specific integrin/ILK signaling pathway. We have solved the NMR structure of the PINCH LIM1 domain and characterized its binding to ILK. LIM1 contains two contiguous zinc fingers of the CCHC and CCCH types and adopts a global fold similar to that of functionally distinct LIM domains from cysteine-rich protein and cysteine-rich intestinal protein families with CCHC and CCCC zinc finger types. Gel-filtration and NMR experiments demonstrated a 1:1 complex between PINCH LIM1 and the ankyrin repeat domain of ILK. A chemical shift mapping experiment identified regions in PINCH LIM1 that are important for interaction with ILK. Comparison of surface features between PINCH LIM1 and other functionally different LIM domains indicated that the LIM motif might have a highly variable mode in recognizing various target proteins.

PubMed: 11078733
DOI: 10.1074/jbc.M007632200

Experimental method

SOLUTION NMR