1FWQ

SOLUTION STRUCTURE OF HUMAN MSS4, A GUANINE NUCLEOTIDE EXCHANGE FACTOR FOR RAB PROTEINS

Summary for 1FWQ

• Entry DOI: 10.2210/pdb1fwq/pdb
• Descriptor: GUANINE NUCLEOTIDE EXCHANGE FACTOR, ZINC ION (2 entities in total)
• Functional Keywords: zinc-binding, beta structure, metal binding protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 13908.15

Authors

Yu, H.,Schreiber, S.L. (deposition date: 2000-09-24, release date: 2000-10-04, Last modification date: 2024-05-22)

Primary citation

Yu, H.,Schreiber, S.L.
Structure of guanine-nucleotide-exchange factor human Mss4 and identification of its Rab-interacting surface.
Nature, 376:788-791, 1995

PubMed Abstract: Guanine-nucleotide-exchange factors (GEFs) promote the exchange of GDP for GTP in Ras GTPases, and thereby positively regulate their functions. Members of the Sec4/Ypt1/Rab branch of the Ras superfamily are essential for vesicular transport. A GEF for a subset of Rab proteins, termed mammalian suppressor of Sec4 (Mss4), has been identified. Here we use multidimensional NMR to determine the structure of human Mss4 (hMss4), which is the first tertiary structure established for a protein with GEF activity. Mss4 contains a central beta-sheet sandwiched between two small sheets. It also binds a Zn2+ ion through Cys 23, Cys 26, Cys 94 and Cys 97. The Rab-binding surface of hMss4 has subsequently been delineated using chemical-shift perturbation experiments and site-directed mutagenesis. The active site of hMss4 involves the Zn(2+)-binding region and a neighbouring loop.

PubMed: 7651540
DOI: 10.1038/376788a0

Experimental method

SOLUTION NMR