1FOS

TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES

1FOS の概要

• エントリーDOI: 10.2210/pdb1fos/pdb
• 分子名称: DNA (5'-D(*AP*AP*TP*GP*GP*AP*TP*GP*AP*GP*TP*CP*AP*TP*AP*GP*GP*AP*GP*A)-3'), DNA (5'-D(*TP*TP*CP*TP*CP*CP*TP*AP*TP*GP*AP*CP*TP*CP*AP*TP*CP*CP*AP*T)-3'), P55-C-FOS PROTO-ONCOGENE PROTEIN, ... (4 entities in total)
• 機能のキーワード: coiled-coil, dna-binding protein, heterodimer, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P01100
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 53934.24

構造登録者

Glover, J.N.M.,Harrison, S.C. (登録日: 1995-03-07, 公開日: 1995-07-10, 最終更新日: 2024-02-07)

主引用文献

Glover, J.N.,Harrison, S.C.
Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA.
Nature, 373:257-261, 1995

PubMed Abstract: The Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous alpha-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.

PubMed: 7816143
DOI: 10.1038/373257a0

実験手法

X-RAY DIFFRACTION (3.05 Å)