1FML
CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP
Summary for 1FML
| Entry DOI | 10.2210/pdb1fml/pdb |
| Related | 1FMJ |
| Descriptor | RETINOL DEHYDRATASE, CALCIUM ION, ADENOSINE-3'-5'-DIPHOSPHATE, ... (5 entities in total) |
| Functional Keywords | sulfotransferase, dehydratase, retinol, adenosine 3', 5'-diphosphate, transferase |
| Biological source | Spodoptera frugiperda (fall armyworm) |
| Total number of polymer chains | 2 |
| Total formula weight | 84618.42 |
| Authors | Pakhomova, S.,Kobayashi, M.,Buck, J.,Newcomer, M.E. (deposition date: 2000-08-17, release date: 2001-05-02, Last modification date: 2024-02-07) |
| Primary citation | Pakhomova, S.,Kobayashi, M.,Buck, J.,Newcomer, M.E. A helical lid converts a sulfotransferase to a dehydratase. Nat.Struct.Biol., 8:447-451, 2001 Cited by PubMed Abstract: We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase. PubMed: 11323722DOI: 10.1038/87617 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.75 Å) |
Structure validation
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